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Objective:To preliminarily study the immune response mediated by inducible costimulation molecule in the role of essential hypertensive renal damage .Methods: The blood pressure of spontaneously hypertensive rats and the wild control group (Wistar-Kyoto rats)was dynamically monitored by noninvasive tail artery blood pressure measuring instrument .The urine protein of the rats in 24 hours was dynamically detected by ELISA .The levels of ICOS protein and its mRNA in the rat′s kidneys were dynamically detected by immunohistochemistry , RT-PCR, respectively.The levels of IL-17A and TGF-β1 in the rat′s plasma and kidneys were dynamically detected by ELISA ,immunohistochemistry ,respectively.The renal pathological changes of the rats were detected by HE and MASSON staining.Results:The blood pressure and urine protein in 24 h of SHRs were significantly higher than that of group WKY from 6 weeks.The expression of ICOS protein and its mRNA in SHRs were significantly higher than that of WKY rats from 6 weeks,and there are significantly positive correlations between the dynamic change of ICOS protein and its mRNA and renal fibrosis score of SHRs (rA=0.813,PA<0.05;rB=0.753,PB<0.05).The expression of IL-17A and TGF-β1 in SHR′s plasma and kidneys were significantly higher than that of WKY rats at the weeks 10 and 23.HE and Masson staining showed that the degree of renal fibrosis of SHRs was sig-nificantly higher than that of WKY rats at 23 weeks.Conclusion:The immune response mediated by ICOS plays an important role in hypertensive renal damage .
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To introduce the example of pouring experiment on frog heart in order to understand the composition,function,fundamentals of work,application and characteristics of the system of Medlab organism signal.It is important to improve the teaching quality of functional experiment in medical college.
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The article elaborates the problems existing in medical education and the necessity of cultivating and innovating teaching method of the young teachers in medical colleges and puts forward the corresponding countermeasures.
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AIM: To study the relationship between renin-angiotensin-aldosterone system and hypertension in coal miners. METHODS: The coal miners received questionnaire investigation and their blood pressure, height and weight were measured, plasma renin activity(PRA), plasma angiotensin Ⅱ(AngⅡ) and aldosterone(ALD) were tested by means of radioimmunoassay in coal miners with hypertension and nor-hypertension. RESULTS: It was found that levels of PRA,AngⅡ and ALD were significantly higher in hypertensive group than in nor-hypertensive group(all P